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bbd24  (MultiTarget Pharmaceuticals)

 
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    MultiTarget Pharmaceuticals bbd24
    Effects of <t>BBD24</t> on proliferation of human osteosarcoma cells and normal blood cells. Cells were treated with BBD24 at indicated concentrations for indicated times. Cell viability was measured by MTT assay. ( A ) IC50 of BBD24 and BBM on human osteosarcoma cells and normal blood cells (peripheral blood mononuclear cells, MBMNCs). ( B , C ) Cell viability of HOS and MG63 after treating with different concentrations of BBD24 and BBM for different times.
    Bbd24, supplied by MultiTarget Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbd24/product/MultiTarget Pharmaceuticals
    Average 90 stars, based on 1 article reviews
    bbd24 - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "2-methylbenzoyl berbamine, a multi-targeted inhibitor, suppresses the growth of human osteosarcoma through disabling NF-κB, ERK and AKT signaling networks"

    Article Title: 2-methylbenzoyl berbamine, a multi-targeted inhibitor, suppresses the growth of human osteosarcoma through disabling NF-κB, ERK and AKT signaling networks

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.103565

    Effects of BBD24 on proliferation of human osteosarcoma cells and normal blood cells. Cells were treated with BBD24 at indicated concentrations for indicated times. Cell viability was measured by MTT assay. ( A ) IC50 of BBD24 and BBM on human osteosarcoma cells and normal blood cells (peripheral blood mononuclear cells, MBMNCs). ( B , C ) Cell viability of HOS and MG63 after treating with different concentrations of BBD24 and BBM for different times.
    Figure Legend Snippet: Effects of BBD24 on proliferation of human osteosarcoma cells and normal blood cells. Cells were treated with BBD24 at indicated concentrations for indicated times. Cell viability was measured by MTT assay. ( A ) IC50 of BBD24 and BBM on human osteosarcoma cells and normal blood cells (peripheral blood mononuclear cells, MBMNCs). ( B , C ) Cell viability of HOS and MG63 after treating with different concentrations of BBD24 and BBM for different times.

    Techniques Used: MTT Assay

    BBD24 down-regulated NF-κB, ERK and AKT signaling pathways of HOS cells. The cells were treated with BBD24 at indicated concentrations for 48 hours, and then nuclear and cytoplasmic proteins were extracted for western blot analysis. β-actin and histone 1 were used as cytoplasmic and nuclear loading controls, respectively. ( A – E ) BBD24 treatment reduced nuclear NF-κB p65 protein level, and inhibited activation of ERK and AKT of HOS cells in dose-dependent manners. Results were expressed as means ± SD of three independent experiments. * P<0.05.
    Figure Legend Snippet: BBD24 down-regulated NF-κB, ERK and AKT signaling pathways of HOS cells. The cells were treated with BBD24 at indicated concentrations for 48 hours, and then nuclear and cytoplasmic proteins were extracted for western blot analysis. β-actin and histone 1 were used as cytoplasmic and nuclear loading controls, respectively. ( A – E ) BBD24 treatment reduced nuclear NF-κB p65 protein level, and inhibited activation of ERK and AKT of HOS cells in dose-dependent manners. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Techniques Used: Protein-Protein interactions, Western Blot, Activation Assay

    BBD24 induced multiple cell death pathways of HOS cells. Human osteosarcoma HOS cells were treated with BBD24 at 2 μg/ml for indicated times. The cells were harvested by trypsinization and collected by centrifugation for analysis of cell viability, apoptosis and necrosis using flow cytometry assay. HOS osteosarcoma cells were treated with BBD24 at the indicated concentrations for 48 hours, followed by Western blot analysis for caspase family and LC3-II. ( A – C ) Flow cytometry assay indicated that BBD24 induced apoptosis and necrosis of HOS cells with time dependence. ( D – I ) BBD24 promoted activation of caspases and autophagy in HOS cells in a dose-dependent manner. Results were expressed as means ± SD of three independent experiments. * P<0.05.
    Figure Legend Snippet: BBD24 induced multiple cell death pathways of HOS cells. Human osteosarcoma HOS cells were treated with BBD24 at 2 μg/ml for indicated times. The cells were harvested by trypsinization and collected by centrifugation for analysis of cell viability, apoptosis and necrosis using flow cytometry assay. HOS osteosarcoma cells were treated with BBD24 at the indicated concentrations for 48 hours, followed by Western blot analysis for caspase family and LC3-II. ( A – C ) Flow cytometry assay indicated that BBD24 induced apoptosis and necrosis of HOS cells with time dependence. ( D – I ) BBD24 promoted activation of caspases and autophagy in HOS cells in a dose-dependent manner. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Techniques Used: Centrifugation, Flow Cytometry, Western Blot, Activation Assay

    BBD24 suppressed OS cells migration and invasion. 1×10 5 MG63 and HOS cells were seeded on a transwell insert, then treating with different concentrations of BBD24. ( A – C ) BBD24 reduced the migration and invasion of MG63 and HOS cells in a dose dependent manner. Results were expressed as means ± SD of three independent experiments. * P<0.05.
    Figure Legend Snippet: BBD24 suppressed OS cells migration and invasion. 1×10 5 MG63 and HOS cells were seeded on a transwell insert, then treating with different concentrations of BBD24. ( A – C ) BBD24 reduced the migration and invasion of MG63 and HOS cells in a dose dependent manner. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Techniques Used: Migration

    BBD24 improved cisplatin sensitivity in vitro . HOS cells were treated with BBD24 and/or cisplatin for 72h, then MTT assay was used to measure the cell viability. ( A , B ) HOS cells were treated with different concentrations of BBD24 or cisplatin. ( C ) HOS cells were treated with BBD24 (2 μg/ml) and/or cisplatin (1 μg/ml). ( D ) Cell viability of HOS in cisplatin group and cisplatin+BBD24 group. ( E , F ) IC50 of BBD24, cisplatin, cisplatin+BBD24 (1 μg/ml) and cisplatin+BBD24 (2 μg/ml). Results were expressed as means ± SD of three independent experiments. * P<0.05.
    Figure Legend Snippet: BBD24 improved cisplatin sensitivity in vitro . HOS cells were treated with BBD24 and/or cisplatin for 72h, then MTT assay was used to measure the cell viability. ( A , B ) HOS cells were treated with different concentrations of BBD24 or cisplatin. ( C ) HOS cells were treated with BBD24 (2 μg/ml) and/or cisplatin (1 μg/ml). ( D ) Cell viability of HOS in cisplatin group and cisplatin+BBD24 group. ( E , F ) IC50 of BBD24, cisplatin, cisplatin+BBD24 (1 μg/ml) and cisplatin+BBD24 (2 μg/ml). Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Techniques Used: In Vitro, MTT Assay

    in vivo anti-tumor activity of BBD24. Nude mice were treated with BBD24 and/or cisplatin for 20 consecutive days. ( A , B ) Tumor volumes in control, cisplatin, BBD24 and BBD24+cisplatin groups. ( C – F ) Immunohistochemical staining of Cleaved-caspase-3, Ki-67 and PCNA in different groups. Results were expressed as means ± SD of three independent experiments. * P<0.05.
    Figure Legend Snippet: in vivo anti-tumor activity of BBD24. Nude mice were treated with BBD24 and/or cisplatin for 20 consecutive days. ( A , B ) Tumor volumes in control, cisplatin, BBD24 and BBD24+cisplatin groups. ( C – F ) Immunohistochemical staining of Cleaved-caspase-3, Ki-67 and PCNA in different groups. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Techniques Used: In Vivo, Activity Assay, Control, Immunohistochemical staining, Staining

    Chemical structure and formula of BBD24.
    Figure Legend Snippet: Chemical structure and formula of BBD24.

    Techniques Used:



    Similar Products

    bbd24  (MultiTarget Pharmaceuticals)
    90
    MultiTarget Pharmaceuticals bbd24
    Effects of <t>BBD24</t> on proliferation of human osteosarcoma cells and normal blood cells. Cells were treated with BBD24 at indicated concentrations for indicated times. Cell viability was measured by MTT assay. ( A ) IC50 of BBD24 and BBM on human osteosarcoma cells and normal blood cells (peripheral blood mononuclear cells, MBMNCs). ( B , C ) Cell viability of HOS and MG63 after treating with different concentrations of BBD24 and BBM for different times.
    Bbd24, supplied by MultiTarget Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbd24/product/MultiTarget Pharmaceuticals
    Average 90 stars, based on 1 article reviews
    bbd24 - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Effects of BBD24 on proliferation of human osteosarcoma cells and normal blood cells. Cells were treated with BBD24 at indicated concentrations for indicated times. Cell viability was measured by MTT assay. ( A ) IC50 of BBD24 and BBM on human osteosarcoma cells and normal blood cells (peripheral blood mononuclear cells, MBMNCs). ( B , C ) Cell viability of HOS and MG63 after treating with different concentrations of BBD24 and BBM for different times.

    Journal: Aging (Albany NY)

    Article Title: 2-methylbenzoyl berbamine, a multi-targeted inhibitor, suppresses the growth of human osteosarcoma through disabling NF-κB, ERK and AKT signaling networks

    doi: 10.18632/aging.103565

    Figure Lengend Snippet: Effects of BBD24 on proliferation of human osteosarcoma cells and normal blood cells. Cells were treated with BBD24 at indicated concentrations for indicated times. Cell viability was measured by MTT assay. ( A ) IC50 of BBD24 and BBM on human osteosarcoma cells and normal blood cells (peripheral blood mononuclear cells, MBMNCs). ( B , C ) Cell viability of HOS and MG63 after treating with different concentrations of BBD24 and BBM for different times.

    Article Snippet: Our findings indicate that BBD24 is a multitarget inhibitor and may represent a new type of anticancer agent for osteosarcoma treatment.

    Techniques: MTT Assay

    BBD24 down-regulated NF-κB, ERK and AKT signaling pathways of HOS cells. The cells were treated with BBD24 at indicated concentrations for 48 hours, and then nuclear and cytoplasmic proteins were extracted for western blot analysis. β-actin and histone 1 were used as cytoplasmic and nuclear loading controls, respectively. ( A – E ) BBD24 treatment reduced nuclear NF-κB p65 protein level, and inhibited activation of ERK and AKT of HOS cells in dose-dependent manners. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Journal: Aging (Albany NY)

    Article Title: 2-methylbenzoyl berbamine, a multi-targeted inhibitor, suppresses the growth of human osteosarcoma through disabling NF-κB, ERK and AKT signaling networks

    doi: 10.18632/aging.103565

    Figure Lengend Snippet: BBD24 down-regulated NF-κB, ERK and AKT signaling pathways of HOS cells. The cells were treated with BBD24 at indicated concentrations for 48 hours, and then nuclear and cytoplasmic proteins were extracted for western blot analysis. β-actin and histone 1 were used as cytoplasmic and nuclear loading controls, respectively. ( A – E ) BBD24 treatment reduced nuclear NF-κB p65 protein level, and inhibited activation of ERK and AKT of HOS cells in dose-dependent manners. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Article Snippet: Our findings indicate that BBD24 is a multitarget inhibitor and may represent a new type of anticancer agent for osteosarcoma treatment.

    Techniques: Protein-Protein interactions, Western Blot, Activation Assay

    BBD24 induced multiple cell death pathways of HOS cells. Human osteosarcoma HOS cells were treated with BBD24 at 2 μg/ml for indicated times. The cells were harvested by trypsinization and collected by centrifugation for analysis of cell viability, apoptosis and necrosis using flow cytometry assay. HOS osteosarcoma cells were treated with BBD24 at the indicated concentrations for 48 hours, followed by Western blot analysis for caspase family and LC3-II. ( A – C ) Flow cytometry assay indicated that BBD24 induced apoptosis and necrosis of HOS cells with time dependence. ( D – I ) BBD24 promoted activation of caspases and autophagy in HOS cells in a dose-dependent manner. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Journal: Aging (Albany NY)

    Article Title: 2-methylbenzoyl berbamine, a multi-targeted inhibitor, suppresses the growth of human osteosarcoma through disabling NF-κB, ERK and AKT signaling networks

    doi: 10.18632/aging.103565

    Figure Lengend Snippet: BBD24 induced multiple cell death pathways of HOS cells. Human osteosarcoma HOS cells were treated with BBD24 at 2 μg/ml for indicated times. The cells were harvested by trypsinization and collected by centrifugation for analysis of cell viability, apoptosis and necrosis using flow cytometry assay. HOS osteosarcoma cells were treated with BBD24 at the indicated concentrations for 48 hours, followed by Western blot analysis for caspase family and LC3-II. ( A – C ) Flow cytometry assay indicated that BBD24 induced apoptosis and necrosis of HOS cells with time dependence. ( D – I ) BBD24 promoted activation of caspases and autophagy in HOS cells in a dose-dependent manner. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Article Snippet: Our findings indicate that BBD24 is a multitarget inhibitor and may represent a new type of anticancer agent for osteosarcoma treatment.

    Techniques: Centrifugation, Flow Cytometry, Western Blot, Activation Assay

    BBD24 suppressed OS cells migration and invasion. 1×10 5 MG63 and HOS cells were seeded on a transwell insert, then treating with different concentrations of BBD24. ( A – C ) BBD24 reduced the migration and invasion of MG63 and HOS cells in a dose dependent manner. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Journal: Aging (Albany NY)

    Article Title: 2-methylbenzoyl berbamine, a multi-targeted inhibitor, suppresses the growth of human osteosarcoma through disabling NF-κB, ERK and AKT signaling networks

    doi: 10.18632/aging.103565

    Figure Lengend Snippet: BBD24 suppressed OS cells migration and invasion. 1×10 5 MG63 and HOS cells were seeded on a transwell insert, then treating with different concentrations of BBD24. ( A – C ) BBD24 reduced the migration and invasion of MG63 and HOS cells in a dose dependent manner. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Article Snippet: Our findings indicate that BBD24 is a multitarget inhibitor and may represent a new type of anticancer agent for osteosarcoma treatment.

    Techniques: Migration

    BBD24 improved cisplatin sensitivity in vitro . HOS cells were treated with BBD24 and/or cisplatin for 72h, then MTT assay was used to measure the cell viability. ( A , B ) HOS cells were treated with different concentrations of BBD24 or cisplatin. ( C ) HOS cells were treated with BBD24 (2 μg/ml) and/or cisplatin (1 μg/ml). ( D ) Cell viability of HOS in cisplatin group and cisplatin+BBD24 group. ( E , F ) IC50 of BBD24, cisplatin, cisplatin+BBD24 (1 μg/ml) and cisplatin+BBD24 (2 μg/ml). Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Journal: Aging (Albany NY)

    Article Title: 2-methylbenzoyl berbamine, a multi-targeted inhibitor, suppresses the growth of human osteosarcoma through disabling NF-κB, ERK and AKT signaling networks

    doi: 10.18632/aging.103565

    Figure Lengend Snippet: BBD24 improved cisplatin sensitivity in vitro . HOS cells were treated with BBD24 and/or cisplatin for 72h, then MTT assay was used to measure the cell viability. ( A , B ) HOS cells were treated with different concentrations of BBD24 or cisplatin. ( C ) HOS cells were treated with BBD24 (2 μg/ml) and/or cisplatin (1 μg/ml). ( D ) Cell viability of HOS in cisplatin group and cisplatin+BBD24 group. ( E , F ) IC50 of BBD24, cisplatin, cisplatin+BBD24 (1 μg/ml) and cisplatin+BBD24 (2 μg/ml). Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Article Snippet: Our findings indicate that BBD24 is a multitarget inhibitor and may represent a new type of anticancer agent for osteosarcoma treatment.

    Techniques: In Vitro, MTT Assay

    in vivo anti-tumor activity of BBD24. Nude mice were treated with BBD24 and/or cisplatin for 20 consecutive days. ( A , B ) Tumor volumes in control, cisplatin, BBD24 and BBD24+cisplatin groups. ( C – F ) Immunohistochemical staining of Cleaved-caspase-3, Ki-67 and PCNA in different groups. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Journal: Aging (Albany NY)

    Article Title: 2-methylbenzoyl berbamine, a multi-targeted inhibitor, suppresses the growth of human osteosarcoma through disabling NF-κB, ERK and AKT signaling networks

    doi: 10.18632/aging.103565

    Figure Lengend Snippet: in vivo anti-tumor activity of BBD24. Nude mice were treated with BBD24 and/or cisplatin for 20 consecutive days. ( A , B ) Tumor volumes in control, cisplatin, BBD24 and BBD24+cisplatin groups. ( C – F ) Immunohistochemical staining of Cleaved-caspase-3, Ki-67 and PCNA in different groups. Results were expressed as means ± SD of three independent experiments. * P<0.05.

    Article Snippet: Our findings indicate that BBD24 is a multitarget inhibitor and may represent a new type of anticancer agent for osteosarcoma treatment.

    Techniques: In Vivo, Activity Assay, Control, Immunohistochemical staining, Staining

    Chemical structure and formula of BBD24.

    Journal: Aging (Albany NY)

    Article Title: 2-methylbenzoyl berbamine, a multi-targeted inhibitor, suppresses the growth of human osteosarcoma through disabling NF-κB, ERK and AKT signaling networks

    doi: 10.18632/aging.103565

    Figure Lengend Snippet: Chemical structure and formula of BBD24.

    Article Snippet: Our findings indicate that BBD24 is a multitarget inhibitor and may represent a new type of anticancer agent for osteosarcoma treatment.

    Techniques: